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KMID : 0191120130280040608
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Journal of Korean Medical Science
2013 Volume.28 No. 4 p.608 ~ p.613
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Chlorogenic Acid Decreases Retinal Vascular Hyperpermeability in Diabetic Rat Model
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Shin Joo-Young
Sohn Joon-Hong
Park Kyu-Hyung
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Abstract
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To evaluate the effect of chlorogenic acid (CGA), a polyphenol abundant in coffee, on retinal vascular leakage in the rat model of diabetic retinopathy, Sprague-Dawley rats were divided into four groups: controls, streptozotocin-induced diabetic rats, and diabetic rats treated with 10 and 20 mg/kg chlorogenic acid intraperitoneally daily for 14 days, respectively. Blood-retinal barrier (BRB) breakdown was evaluated using FITC-dextran. Vascular endothelial growth factor (VEGF) distribution and expression level was evaluated with immunohistochemistry and Western blot analysis. Expression of tight junction proteins, occludin and claudin-5, and zonula occludens protein, ZO-1 was also evaluated with immunohistochemistry and Western blot analysis. BRB breakdown and increased vascular leakage was found in diabetic rats, with increased VEGF expression and down-regulation of occludin, claudin-5, and ZO-1. CGA treatment effectively preserved the expression of occludin, and decreased VEGF levels, leading to less BRB breakdown and less vascular leakage. CGA may have a preventive role in BRB breakdown in diabetic retinopathy by preserving tight junction protein levels and low VEGF levels.
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KEYWORD
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Chlorogenic Acid, Diabetic Retinopathy, Diabetes Rat Model, Vascular Endothelial Growth Factor, Blood-Retinal Barrier
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